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ORIGINAL ARTICLE
Year : 2013  |  Volume : 1  |  Issue : 1  |  Page : 51-56

Evaluation of the different irrigation regimens with sodium hypochlorite and EDTA in removing the smear layer during root canal preparation


1 Department of Semiology and Clinics, School of Dentistry, Federal University of Pelotas, Pelotas, RS, Brazil
2 Laboratory of Immunology and Microscopy, EMBRAPA-CPACT, Pelotas, RS, Brazil

Correspondence Address:
Luiz Fernando Machado Silveira
Federal University of Pelotas, School of Dentistry, Department of Semiology and Clinics, Gonçalves Chaves St., 457 – Centro, Pelotas, RS, CEP 96015-560
Brazil
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Source of Support: None, Conflict of Interest: None


DOI: 10.1016/j.jmau.2013.06.003

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Objectives: The aim of this study was to compare, by scanning electron microscopy analysis, the cleaning efficacy of a 2.5% sodium hypochlorite (NaOCl) and a 17% ethylenediaminetetra-acetic acid (EDTA) solution with the two solutions either applied alternately or mixed together for smear layer removal after the use of each endodontic file in different root thirds. Materials and methods: Fifty-four single-rooted human maxillary premolars were used and divided into three groups. Manual instrumentation was performed with K-Flexofiles with the crown-down technique; and divided in Group 1: canal preparation was performed with 2.5% NaOCl mixed with 17% EDTA in the root canal. Group 2: irrigation was performed alternately with 2.5% NaOCl and 17% EDTA. Group 3: only 2.5% NaOCl was used during all instrumentation and EDTA for 3 min at the final. The mean scores for the smear layer by SEM after the use of each file were calculated and analysed. Results: A statistically significant difference (P < 0.05) was found among the instrumentation groups between the apical third and the middle and coronal thirds. In the apical third, the canal walls were often contaminated by inorganic debris and smear layer. Conclusions: The alternate or mixed use of EDTA during instrumentation with 2.5% sodium hypochlorite was the most effective form of irrigation for the removal of smear layer on the cervical and middle thirds. No form of irrigation was sufficiently effective to remove the smear layer in the apical third. Clinical relevance: The importance of the alternating use of 17% EDTA and 2.5% sodium hypochlorite during root canal instrumentation.


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