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EXPERIMENTAL STUDY
Year : 2016  |  Volume : 4  |  Issue : 1  |  Page : 28-35

Microscopic study of the morphology and metabolic activity of Fusarium oxysporum f. sp. gladioli treated with Jatropha curcas oil and derivatives


1 Instituto Politécnico Nacional, Centro de Desarrollo de Productos Bióticos. Km. 6 Carr. Yautepec-Jojutla, Col. San Isidro, CEPROBI 8, Yautepec, Morelos. C.P. 62731, México
2 Jardín Botánico, Instituto de Biología, Universidad Nacional Autónoma de . Ciudad Universitaria. C.P. 04510, México
3 Centro de Investigaciones Químicas, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Col. Chamilpa, 62209 Cuernavaca, Morelos, México

Correspondence Address:
S Bautista-Baños
Instituto Politécnico Nacional, Centro de Desarrollo de Productos Bióticos. Km. 6 Carr. Yautepec-Jojutla, Col. San Isidro, CEPROBI 8, Yautepec, Morelos. C.P. 62731
México
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Source of Support: None, Conflict of Interest: None


DOI: 10.1016/j.jmau.2015.10.004

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The fungus Fusarium oxysporum f. sp. gladioli is one of the main pathogenic microorganisms of the ornamental genus Gladiolus. The attack of this microorganism includes corms and different plant phenological stages. In this study, different microscopic techniques and fluorochromes were used to evaluate the effect of J. curcas oil and acylglycerides, namely trilinolein, triolein, monomyristin and dimyristin, on the morphology, membrane integrity (%), viability (%) and germination (%) of F. oxsporum f sp. gladioli. Phase-contrast optical photomicrographs and scanning microscopy showed that J. curcas oil and the triglycerides triolein and trilinolein caused the formation of numerous vacuoles, alterations in the morphology of the outer covering of the mycelium and conidia, and inhibition of membrane activity in the fungus during 24 h of incubation. The fluorochromes used detected no permanent damage to the viability of the conidia. The high germination percentage of the conidia of Fusarium oxysporum f. sp. gladioli indicates that the damage caused by the application of the treatments was fungistatic rather than fungicidal and did not cause cell death.


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