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ORIGINAL ARTICLE
Year : 2018  |  Volume : 6  |  Issue : 2  |  Page : 93-98

Pathological changes in experimental intramammary infection with different Staphylococcus species in mice


1 Department of Veterinary Pathology, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University; Patho-Epidemiology Laboratory, Indian Council of Agricultural Research-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India
2 Department of Veterinary Pathology, Veterinary College, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka, India
3 Patho-Epidemiology Laboratory, Indian Council of Agricultural Research-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India

Correspondence Address:
Dr. P Krishnamoorthy
Indian Council of Agricultural Research-National Institute of Veterinary Epidemiology and Disease Informatics, Post Box No. 6450, Ramagondanahalli, Yelahanka, Bengaluru - 560 064, Karnataka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/JMAU.JMAU_21_18

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Introduction: Mastitis is caused by different Staphylococcus species, produce great economic loss to farmers. Present study was conducted to know pathological changes in mice inoculated with Staphylococcus epidermidis, S. chromogenes, S. haemolyticus and S. aureus isolated from bovine milk. Materials and Methods: Mice were inoculated with 50 μl (2x104 cfu organisms) per mammary gland and euthanized at 6, 12, 24, 48, 72 and 96 h. Mammary gland weight, gross and histopathological changes of mammary gland, liver, kidney, spleen, heart, lung and inguinal lymph node were studied. Results: Mammary gland weight and percentage of body weight increased at 6 and 96 h in S. aureus and S. haemolyticus infected mice. Gross changes were observed in mammary gland but not in other organs. Mammary gland revealed gross changes from 24 to 72 h in three Coagulase negative staphylococcal (CNS) species and persisted up to 96 h in S. aureus infected mice. Histopathological changes in mammary glands was severe in S. aureus and moderate in CNS species. S. aureus infected mice revealed severe damage to alveoli and loss of alveolar architecture at 96 h but three CNS species infection was overcome by host factors which was evident by proliferation of alveolar epithelial cells. No histological changes were observed in kidney, spleen, lung, heart and inguinal lymph nodes. Conclusions: S. aureus caused severe mastitis in mice when compared to CNS species. Further, it is first report of mice to study CNS mastitis, and in future it can be used as model for CNS mastitis.


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