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Year : 2018  |  Volume : 6  |  Issue : 4  |  Page : 197-204

Histological study on the protective effect of endogenous stem cell mobilization in busulfan-induced testicular injury in albino rats

Department of Medical Histology and Cell Biology, Faculty of Medicine, Cairo University, Cairo, Egypt

Correspondence Address:
Dr. Dalia Ibrahim Ismail
4 Al-Falaky Street, Kasr Al-Ainy, Cairo
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/JMAU.JMAU_35_18

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Background: Testicular damage is one of the most hazardous effects of chemotherapy as it is frequently associated with oligozoospermia and azoospermia. Aim of the Work: This study aimed at evaluating the protective effect of hematopoietic stem cell mobilization by granulocyte colony-stimulating factor (G-CSF) in a rat model of busulfan-induced testicular injury. Materials and Methods: Twenty-four adult albino rats were divided into four groups: group I, the control, Group II: rats received two doses of busulfan (each 15 mg/kg) intraperitoneally (IP) with 14 days interval, Group III: rats received busulfan and left untreated, and Group IV received busulfan IP then G-CSF (70 μg/kg/day) subcutaneously for 5 consecutive days. Testicular sections were stained with H and E and immunohistochemically for CD34, proliferating cell nuclear antigen (PCNA) and caspase-3, and semithin sections were stained with toluidine blue. Results: Groups II and III showed loss of the normal histological architecture of the testis and spermatogenic cells, with increased apoptosis confirmed by significantly increased caspase-3 and significantly decreased PCNA immunoexpression. While Group IV revealed improved testicular histology, decreased apoptosis, and increased proliferative capacity of spermatogenic cells. This was confirmed by significantly decreased caspase-3 immunoexpression and increased PCNA immunoreaction. Conclusion: Mobilization of stem cells with G-CSF was found to improve the testicular histology following busulfan chemotherapy in albino rats.

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