• Users Online: 504
  • Print this page
  • Email this page
Year : 2019  |  Volume : 7  |  Issue : 1  |  Page : 28-43

Cytotoxic effects of extract of Acmella oleracea in the ovaries and midgut of Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) female ticks

1 Graduate Program in Chemistry, Universidade Federal do Maranhão (UFMA), Av. dos Portugueses, 1966, Bacanga, São Luís/MA, Paulínia, São Paulo, Brazil
2 Division of Chemistry of Natural Products, CPQBA/UNICAMP, Paulínia, São Paulo, Brazil
3 Graduate Program in Animal Science, School of Agricultural Science and Veterinary Medicine, The Pontificia Universidade Catolica do Parana – PUCPR, Rua Imaculada Conceição, Curitiba, PR, Brazil
4 Embrapa Meio-Norte, Parnaíba, PI, Brazil

Correspondence Address:
Dr. Patrícia Rosa de Oliveira
Department of Biology, Institute of Biosciences, São Paulo State University-UNESP, Av. 24 A, n° 1515, Postal Code 199, Zip Code 13506-900, Rio Claro, São Paulo
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/JMAU.JMAU_16_18

Get Permissions

The present study investigated the effects of different concentrations of Acmella oleracea extract on the germinative cells and digestive processes of semi-engorged Rhipicephalus sanguineus females. For this experiment, 150 ticks were divided into five groups (30 individuals each). The animals were immersed for 5 min in different concentrations of the extract, distilled water, or ethanol 50%/DMSO 1%, dried, and kept in biological oxygen demand incubator for 7 days. The alterations were associated with the size of germinative cells and yolk granules; presence, size, and location of vacuoles in the cytoplasm of germinative cells; nuclear modifications in the germinative cells; damages to the nucleus and cytoplasm of the midgut generative cells; size of digestive cells; number of captured blood elements; accumulated digestive residues and digestive vacuoles in the digestive cells cytoplasm; and the number and distribution of proteins and polysaccharides in all the cells of both organs. The concentrations used in this study prevented an efficient and complete blood digestion by the midgut epithelial cells of the treated animals, resulting in the absence of the necessary nutrients to maintain the physiological events in the ectoparasites. In advanced stages, This can lead the ectoparasite to death. The germinative cells were highly impaired and probably not able to advance developmental stages (I–V) or complete vitellogenesis to be released during ovulation, which would prevent the females from originating a new individual. Thus, it can be concluded that the effects of A. oleracea are similar to those caused by chemical products widely recognized as effective to control ticks.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded211    
    Comments [Add]    
    Cited by others 2    

Recommend this journal