• Users Online: 509
  • Print this page
  • Email this page
ORIGINAL ARTICLE
Year : 2020  |  Volume : 8  |  Issue : 3  |  Page : 104-114

Qualitative and quantitative study of the changes in the ultrastructure of mammalian adrenal cortex caused by the venezuelan tigra mariposa (Bothrops venezuelensis) snake venom


1 Microscopy Electronic Centre, Faculty of Sciences, Universidad Central De Venezuela, Caracas, Venezuela
2 Anatomical Institute, Immunochemistry and Ultrastructure Laboratory, Universidad Central De Venezuela, Caracas, Venezuela

Correspondence Address:
Dr. Alexis Rodriguez-Acosta
Immunochemistry and Ultrastructure Laboratory, Anatomical Institute, Universidad Central De Venezuela, Caracas
Venezuela
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/JMAU.JMAU_49_19

Get Permissions

The damage of the adrenal gland by snake venoms needs to be clarified. Lethality (LD50) of Bothrops venezuelensis (Bv) venom was established by intraperitoneally mice injections. Preparation of specimens for transmission electron microscopy samples from cortex adrenal gland biopsies at 3, 6, and 24 h was processed. The quantitative description by the principal component analysis (PCA) of the adrenal gland was as follows: thickening of the capillary endothelium, area of the capillary lumen, cell nucleus area, enlargement of the perinuclear space, number of mitochondria, area of the mitochondria, number of mitochondrial cristae, number of cristae per mitochondrial unit, and tubular diameter of the smooth endoplasmic reticulum (SER). Sections of the adrenal cortex, after 3 h postinjection with Bv venom showed in the cortical cells: mitochondria with tubular cristae and slightly swollen SER cisternae, nucleus with variable heterochromatin content, irregular edges, and swollen nuclear envelope. After 6 h, cells with swollen nucleus envelope, electron dense lipids and mitochondria with loss of their cristae were observed. Myelin figures, close to the microvilli of the cortical cell, multivesicular bodies, swollen profiles of the SER, and electron dense lipid drops were noticed. After 24 h, thickening of the endothelial wall, fenestrae and projections into the capillary lumen, loss of the mitochondrial cristae, destruction of the capillary and the plasma membrane of the cortical cell, multivesicular body, SER loss, and an enlargement of the perinuclear space were detected. In the quantitative PCA, there were significant changes after the venom treatments.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed108    
    Printed5    
    Emailed0    
    PDF Downloaded28    
    Comments [Add]    

Recommend this journal