Journal of Microscopy and Ultrastructure

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 7  |  Issue : 1  |  Page : 28--43

Cytotoxic effects of extract of Acmella oleracea in the ovaries and midgut of Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) female ticks


Patrícia Rosa de Oliveira1, Luis Adriano Anholeto1, Rodney Alexandre Ferreira Rodrigues2, André Arnosti1, Gervásio Henrique Bechara3, Karina Neoob de Carvalho Castro4, Maria Izabel Camargo-Mathias1 
1 Graduate Program in Chemistry, Universidade Federal do Maranhão (UFMA), Av. dos Portugueses, 1966, Bacanga, São Luís/MA, Paulínia, São Paulo, Brazil
2 Division of Chemistry of Natural Products, CPQBA/UNICAMP, Paulínia, São Paulo, Brazil
3 Graduate Program in Animal Science, School of Agricultural Science and Veterinary Medicine, The Pontificia Universidade Catolica do Parana – PUCPR, Rua Imaculada Conceição, Curitiba, PR, Brazil
4 Embrapa Meio-Norte, Parnaíba, PI, Brazil

Correspondence Address:
Dr. Patrícia Rosa de Oliveira
Department of Biology, Institute of Biosciences, São Paulo State University-UNESP, Av. 24 A, n° 1515, Postal Code 199, Zip Code 13506-900, Rio Claro, São Paulo
Brazil

The present study investigated the effects of different concentrations of Acmella oleracea extract on the germinative cells and digestive processes of semi-engorged Rhipicephalus sanguineus females. For this experiment, 150 ticks were divided into five groups (30 individuals each). The animals were immersed for 5 min in different concentrations of the extract, distilled water, or ethanol 50%/DMSO 1%, dried, and kept in biological oxygen demand incubator for 7 days. The alterations were associated with the size of germinative cells and yolk granules; presence, size, and location of vacuoles in the cytoplasm of germinative cells; nuclear modifications in the germinative cells; damages to the nucleus and cytoplasm of the midgut generative cells; size of digestive cells; number of captured blood elements; accumulated digestive residues and digestive vacuoles in the digestive cells cytoplasm; and the number and distribution of proteins and polysaccharides in all the cells of both organs. The concentrations used in this study prevented an efficient and complete blood digestion by the midgut epithelial cells of the treated animals, resulting in the absence of the necessary nutrients to maintain the physiological events in the ectoparasites. In advanced stages, This can lead the ectoparasite to death. The germinative cells were highly impaired and probably not able to advance developmental stages (I–V) or complete vitellogenesis to be released during ovulation, which would prevent the females from originating a new individual. Thus, it can be concluded that the effects of A. oleracea are similar to those caused by chemical products widely recognized as effective to control ticks.


How to cite this article:
de Oliveira PR, Anholeto LA, Ferreira Rodrigues RA, Arnosti A, Bechara GH, de Carvalho Castro KN, Camargo-Mathias MI. Cytotoxic effects of extract of Acmella oleracea in the ovaries and midgut of Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) female ticks.J Microsc Ultrastruct 2019;7:28-43


How to cite this URL:
de Oliveira PR, Anholeto LA, Ferreira Rodrigues RA, Arnosti A, Bechara GH, de Carvalho Castro KN, Camargo-Mathias MI. Cytotoxic effects of extract of Acmella oleracea in the ovaries and midgut of Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) female ticks. J Microsc Ultrastruct [serial online] 2019 [cited 2020 Jul 5 ];7:28-43
Available from: http://www.jmau.org/article.asp?issn=2213-879X;year=2019;volume=7;issue=1;spage=28;epage=43;aulast=de;type=0