Figure 2: Histopathological photomicrographs of liver of Heteropneustes fossilis under control conditions (Co), glyphosate-treated laboratory conditions (GL), and glyphosate-treated field conditions (GF). (A) Normal appearance of hepatocytes (HC) and compact arrangement around central vein (CV) with distinct nucleus (N) under light microscopy (Co, 1000×). (B) Hypertrophied and pyknotic nuclei (white arrow), vacuolation in cytoplasm of hepatocytes (broken arrow) under light microscopy (GL, 1000×). (C) Light microscopy showing vacuolation in cytoplasm (broken arrow) and appearance of sinusoidal space (oval) (GF, 1000×). (D) Normal appearance of hepatocytes with large number of mitochondria (M), rough endoplasmic reticulum (RER) and glycogen droplets (GY) under TEM (Co, 6300×). (E) Hepatocytes showing necrotic nucleus (arrow), vacuolation in cytoplasm (broken arrow) with reduced amount of RER under TEM (GL, 2500×). (F) TEM showing hepatocytes with dilated mitochondria (bold arrow) and cytoplasmic vacuolation (broken arrow; GF, 5000× ). TEM = transmission electron microscopy.