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  Indian J Med Microbiol
 

Figure 4: A photomicrograph of a longitudinal section in a rat diaphragm muscle of experimental Group II (nicotine only), showing some fibers with pale sarcoplasm and crowded nuclei (↑). Some of the peripheral-flattened nuclei are pyknotic (▴), and striation is lost in some muscular tissues. Notice centrally located nuclei (*) with appearance of splitting of the muscle fibers (↑↑) (H& E × 40)

Figure 4: A photomicrograph of a longitudinal section in a rat diaphragm muscle of experimental Group II (nicotine only), showing some fibers with pale sarcoplasm and crowded nuclei (↑). Some of the peripheral-flattened nuclei are pyknotic (▴), and striation is lost in some muscular tissues. Notice centrally located nuclei (*) with appearance of splitting of the muscle fibers (↑↑) (H& E × 40)