• Users Online: 37
  • Print this page
  • Email this page
ORIGINAL ARTICLE
Year : 2015  |  Volume : 3  |  Issue : 1  |  Page : 38-43

Ultrastructural effect on mastitis pathogens by extract of endophytic fungi associated with ethnoveterinary plant, Hibiscus sabdariffa L.


Microbiology Laboratory, Department of Biotechnology & Bioinformatics, North-Eastern Hill University, Shillong 793 022, Meghalaya, India

Correspondence Address:
S R Joshi
Microbiology Laboratory, Department of Biotechnology & Bioinformatics, North-Eastern Hill University, Shillong 793 022, Meghalaya
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.1016/j.jmau.2014.10.001

Get Permissions

Three endophytic fungi isolated from different parts of Hibiscus sabdariffa L. were identified using morphological and molecular approaches. Ethanolic extract of endophytic fungi as well as plant extracts were evaluated for in vitro antibacterial activity by using well diffusion method and their minimum inhibitory concentration estimated. The culture extract of one endophytic fungus Glomerella acutata EF15 was found to be potent antibacterial agent against pathogenic coliform bacteria Klebsiella pneumoniae and Escherichia coli responsible for causing clinical mastitis. Scanning electron microscopy (SEM) revealed the ultrastructural alteration in the cells of K. pneumoniae and E. coli when treated with crude ethanolic extract of G. acutata. The ethanolic extract of the endophytic fungus revealed potential to be bioprospected as antibacterial agent against pathogens causing coliform mastitis, the existing havoc of dairy industries.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1243    
    Printed40    
    Emailed0    
    PDF Downloaded120    
    Comments [Add]    
    Cited by others 2    

Recommend this journal