Journal of Microscopy and Ultrastructure

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 2  |  Issue : 3  |  Page : 161--176

Alpha-chlorohydrin effects on the epididymis of adult albino rat: A histological and immunohistochemical study


Gehan Soliman, Sadika M.T. Al Ebs, Amal A.A. Abd-El-Hafez 
 Tanta University, Faculty of Medicine, Histology Department, Egypt

Correspondence Address:
Amal A.A. Abd-El-Hafez
Tanta University, Faculty of Medicine, Histology Department
Egypt

α-Chlorohydrin (ACH) is a well-known food contaminant, has shown anti-fertility activity in males. In this research we studied the histological and immunohistochemical changes in the epididymis of adult albino rat. Twenty adult male albino rats were divided into control (group 1) (10 rats) and experimental (groups 2 and 3) (10 rats). The experimental rats received α-Chlorohydrin at a single oral dose of 100 mg/kg body weight. After 6 h (group 2) and 24 h (group 3) of ACH treatment, the specimens were submitted for the light, electron microscopic and β-tubulin immunohistochemical evaluations. Light microscopy after 6 h showed focal separation of lining epithelium, while EM examinations showed dilated rER, and Golgi complex. After 24 h LM demonstrated different stages of cell exfoliation; however, EM proved multilamellar bodies and dilated rER besides the granules. Immunohistochemi- cal examinations revealed decreased reactions in experimental groups. However, the mean total optical density of the β-tubulin immunostaining revealed statistical nonsignificant difference between the three groups. Thus ACH as an undesirable unavoidable food contaminant induced rapid histological alterations on caput epididymis with duration-dependent manner at the level of the cell and tissue, that puts another obstacle on the road of fertility.


How to cite this article:
Soliman G, Al Ebs SM, Abd-El-Hafez AA. Alpha-chlorohydrin effects on the epididymis of adult albino rat: A histological and immunohistochemical study.J Microsc Ultrastruct 2014;2:161-176


How to cite this URL:
Soliman G, Al Ebs SM, Abd-El-Hafez AA. Alpha-chlorohydrin effects on the epididymis of adult albino rat: A histological and immunohistochemical study. J Microsc Ultrastruct [serial online] 2014 [cited 2021 Apr 15 ];2:161-176
Available from: https://www.jmau.org/article.asp?issn=2213-879X;year=2014;volume=2;issue=3;spage=161;epage=176;aulast=Soliman;type=0