Journal of Microscopy and Ultrastructure

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 9  |  Issue : 1  |  Page : 26--30

Aesculus hippocastanum-derived extract β-Aescin and in vitro antibacterial activity


Salma L Dahash, Ourba K Abass, Myada M Abdul-Razaq, Hayder M Al-Kuraishy, Ali I Al-Gareeb 
 Department of Medical Microbiology, College of Medicine, Almustansiriya University, Baghdad, Iraq

Correspondence Address:
Dr. Hayder M Al-Kuraishy
Department of Pharmacology, Toxicology and Medicine, College of Medicine, Almustansiriya University, P.O. Box: 14132, Baghdad
Iraq

Objectives: The objective of this study was to investigate the antibacterial activity of β-aescin against common Gram-negative and Gram-positive bacteria. Materials and Methods: Agar well diffusion assay was used to determine the antibacterial activity of β-aescin against common Gram-negative and Gram-positive bacteria including Klebsiella pneumoniae, Escherichia coli, Staphylococcus epidermidis, Staphylococcus aureus, and Pseudomonas aeruginosa. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of β-aescin were evaluated by serial dilution method. Results: β-aescin led to significant antibacterial effects on the tested Gram-negative and Gram-positive bacteria compared to the negative control, P < 0.05 for K. pneumoniae and P. aeruginosa and P < 0.01 for E. coli, S. epidermidis, and S. aureus. On the other hand, β-aescin produced a comparable less antibacterial effect on K. pneumoniae, E. coli, and P. aeruginosa compared to the positive control, P < 0.01, whereas β-aescin illustrated a comparable effect with that of the positive control on Gram-positive S. epidermidis, P = 0.05. Furthermore, β-aescin illustrated a concentration-dependent antibacterial effect against Gram-positive S. epidermidis and S. aureus compared to the different concentrations, P < 0.01. MIC and MBC of β-aescin were high for Gram-negative bacteria and low for Gram-positive bacteria compared to MIC of the positive control. Conclusions: β-aescin is an effective antibacterial herb mainly against Gram-positive S. epidermidis and S. aureus in a concentration-dependent manner.


How to cite this article:
Dahash SL, Abass OK, Abdul-Razaq MM, Al-Kuraishy HM, Al-Gareeb AI. Aesculus hippocastanum-derived extract β-Aescin and in vitro antibacterial activity.J Microsc Ultrastruct 2021;9:26-30


How to cite this URL:
Dahash SL, Abass OK, Abdul-Razaq MM, Al-Kuraishy HM, Al-Gareeb AI. Aesculus hippocastanum-derived extract β-Aescin and in vitro antibacterial activity. J Microsc Ultrastruct [serial online] 2021 [cited 2021 Apr 11 ];9:26-30
Available from: https://www.jmau.org/article.asp?issn=2213-879X;year=2021;volume=9;issue=1;spage=26;epage=30;aulast=Dahash;type=0